Fig. 1

Ss-CAD-silenced transformants showing reduced Ss-CAD expression levels and CAD activity. aSs-CAD cDNA expression levels were measured by qRT-PCR and were normalized to that of actin cDNA in extracts taken from each developmental stage. cDNA abundances from samples grown on PDA for 2 d were assigned a value of 1.0. For all experiments, three independent replicates were performed. b qRT-PCR analysis of Ss-CAD transcript accumulation in Ss-CAD-silenced transformants. Ss-CAD expression in the wild-type strain Ep-1PNA367 served as control. Hyphal masses from 3-day-old colonies on PDA were collected for gene expression analysis. c CAD activity in the wild-type strain and Ss-CAD-silenced transformants was measured by enzyme activity assays. CAD activity in the wild-type strain was assigned a value of 1.0. Three independent replicates were performed for all experiments. Values are means ± S.E. **P < 0.01; one-way ANOVA