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Table 1 Characteristics of different types of CRISPR/Cas systems involved in plant-pathogen interactions

From: Applications of CRISPR technology in studying plant-pathogen interactions: overview and perspective

CRISPR system Cas9 Cas12a Cas12b Cas13 Cas14
Type and Class Type II
Class 2 		Type V
Class 2 		Type V
Class 2 		Type VI
Class 2 		Type V
Class 2
Pre-crRNA processing No Yes No Yes Yes
tracrRNA Yes No Yes No Yes
PAM/PFS 3′, G-rich 5′, T-rich 5′, T-rich 3′, non-G PFS Independent-PAM
Nuclease domain RuvC, HNH RuvC RuvC HPEN RuvC
Substrate dsDNA dsDNA dsDNA RNA ssDNA
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Phytopathology Research

ISSN: 2524-4167

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