From: Applications of CRISPR technology in studying plant-pathogen interactions: overview and perspective
CRISPR system | Cas9 | Cas12a | Cas12b | Cas13 | Cas14 |
---|---|---|---|---|---|
Type and Class | Type II Class 2 | Type V Class 2 | Type V Class 2 | Type VI Class 2 | Type V Class 2 |
Pre-crRNA processing | No | Yes | No | Yes | Yes |
tracrRNA | Yes | No | Yes | No | Yes |
PAM/PFS | 3′, G-rich | 5′, T-rich | 5′, T-rich | 3′, non-G PFS | Independent-PAM |
Nuclease domain | RuvC, HNH | RuvC | RuvC | HPEN | RuvC |
Substrate | dsDNA | dsDNA | dsDNA | RNA | ssDNA |