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Table 1 Characteristics of different types of CRISPR/Cas systems involved in plant-pathogen interactions

From: Applications of CRISPR technology in studying plant-pathogen interactions: overview and perspective

CRISPR system

Cas9

Cas12a

Cas12b

Cas13

Cas14

Type and Class

Type II

Class 2

Type V

Class 2

Type V

Class 2

Type VI

Class 2

Type V

Class 2

Pre-crRNA processing

No

Yes

No

Yes

Yes

tracrRNA

Yes

No

Yes

No

Yes

PAM/PFS

3′, G-rich

5′, T-rich

5′, T-rich

3′, non-G PFS

Independent-PAM

Nuclease domain

RuvC, HNH

RuvC

RuvC

HPEN

RuvC

Substrate

dsDNA

dsDNA

dsDNA

RNA

ssDNA