Fig. 2
Antisera titer determination by western blotting. All antisera were used at 10 different dilutions (1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000 and 1:512000). Each panel consists of a left lane of pageruler prestained protein ladder (Marker), a middle lane containing total protein extracted from N. benthamiana leaves, transiently expressing MPCABYV (a), MPMABYV (b) and MPSABYV (c) proteins. pMDC32 empty vector was used as a negative control in the right lane