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Table 1 Sequences of the optimal primers and probe for the RPA assay

From: Development and evaluation of a recombinase polymerase amplification assay for rapid detection of strawberry red stele pathogen

Primer/ probe

Name

Sequence (5′-3′)

Forward primer

Phy_Gen_F3

TGATGGCTTTCTTAATTTTATTTGCTTTTTA

Reverse Primer

Phy_Frag_R29

TGTTTGAAAAGAGCTAATTACGTATTAAATAT

TwistAmp exo probe

Phy_Frag_RevP3

AATTACGTATTAAATATACATATATATC-T(ROX)-A-(dSpacer)-T(BHQ2)-ACGAGATTAATATAAT[3′-C3SPACER]