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Fig. 1 | Phytopathology Research

Fig. 1

From: Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype

Fig. 1

Steps for obtaining single-spore isolates of MoT. a Collection of infected wheat spike. b Incubation of infected wheat neck for sporulation. c Microscopic view of sporulation of blast conidia after overnight incubation. d Touching conidia with a dissecting needle tip. e Transferring conidia on needle tip to PDA medium. f Spreading conidia in plate by a round-end glass rod. g Germination of a single conidium. h A single germinated conidia pointed by a needle (black U-shaped) under microscope. i Placing the single germinated conidia on PDA plate. j Purified isolate from a single conidium. Arrow heads indicate germinated conidia under microscope (g and h)

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