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Fig. 2 | Phytopathology Research

Fig. 2

From: Suitable methods for isolation, culture, storage and identification of wheat blast fungus Magnaporthe oryzae Triticum pathotype

Fig. 2

Steps of filter paper storage method used for preservation of MoT isolates. a Preparation of small pieces (2–3 cm) of sterilized filter paper. b Placing filter paper on PDA plate. c Placing fungal block at the centre of PDA plate. d Growth of MoT isolate (before overlapping with filter paper pieces). e Growth of MoT isolate (after overlapping with filter paper pieces). f Filter paper pieces with mycelia in a conical flask containing silica gel. g Drying of filter paper in a desiccator. h Storing of filter paper pieces in microcentrifuge tube at − 20 °C

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