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Table 2 Level and stability of pyraclostrobin resistance in PR mutants and their parental isolates of Valsa mali

From: Baseline sensitivity and resistance risk assessment of Valsa mali to pyraclostrobin

Isolate

Origin

EC50 (μg/mL)

RF

1st generation

10th generation

1st generation

10th generation

XJVM001

Parent

0.0033 d

0.0030 d

XJVM001R1

PR mutant

0.1350 b

0.1020 b

41.0

33.9

XJVM001R2

PR mutant

0.1870 a

0.1580 a

56.8

52.5

972

Parent

0.0051 d

0.0049 d

972R

PR mutant

0.1120 c

0.0575 c

22.0

11.7

  1. EC50 = effective concentration for 50% inhibition of mycelial growth; RF (resistance factor) = EC50 for the resistant mutant/EC50 for the sensitive parental isolate; PR mutants were obtained by growing parental isolates on pyraclostrobin-amended medium. PR mutants are listed below their respective parents. Multiple range test was used to compare EC50 values using Fisher’s least significant difference test. Values followed by different letters are significantly different at P < 0.05