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Fig. 2 | Phytopathology Research

Fig. 2

From: FpCzf14 is a putative C2H2 transcription factor regulating conidiation in Fusarium pseudograminearum

Fig. 2

Construction of FpCzf14-deleted mutant and complementation strain, and subcellular localization of FpCzf14. a Schematic representation of the FpCzf14 deletion strategy and PCR amplification. b PCR products for the FpCzf14 gene replacement construct. M, DNA size marker; H, a 750-bp fragment of the hygromycin phosphotransferase gene (hph); G, a 806-bp fragment of the FpCzf14 gene; F, a 1479-bp fragment of FpCZF14 and hph upstream cassette; R, a 1691-bp fragment of FpCZF14 and hph downstream cassette. c Southern blot analysis of WT and Δfpczf14-T1 with a 1027-bp fusion fragment containing 562-bp FpCzf14 upstream sequence and 465-bp hph as a probe. The genomic DNA of each strain was digested with Xba I. d The expression and localization of FpCzf14-GFP in Δfpczf14 mutant using its native promoter. The nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) and examined by fluorescence microscopy. DIC: differential interference contrast

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