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Fig. 2 | Phytopathology Research

Fig. 2

From: Rust effector PNPi interacting with wheat TaPR1a attenuates plant defense response

Fig. 2

Subcellular localization of TaPR1a and SP(TaPR1a)-PNPi(23–333) in N. benthamiana epidermal cells. a The TaPR1a(1–164)-RFP recombinant segment was transiently expressed in N. benthamiana leaves by agroinfiltration. b SP(TaPR1a)-PNPi(23–333)-GFP was transiently expressed in tobacco leaves. RFP or GFP alone was used as a control. Leaf epidermal peels were plasmolyzed (white arrows) by incubation in 800 mM mannitol for 6 min. BF, bright field; EV, empty vector; RFP, red fluorescent protein; GFP, green fluorescent protein. Scale bar = 100 μm

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