Fig. 3
From: Rust effector PNPi interacting with wheat TaPR1a attenuates plant defense response
The interaction of PNPi and TaPR1a proteins was detected by BiFC assay. YFP signals were detected in the leaves of N. benthamiana co-expressing SP(TaPR1a)-PNPi(23–333)-YFPN and TaPR1a-YFPC. Co-expression of AvrLm1-YFPN and BnMPK9-YFPC was utilized as a positive control. Co-expression of each recombinant vector with the corresponding TaPR1a signal peptide-fused YFPN and YFPC vectors served as negative controls. BF, bright field; EV, empty vector; YFP, yellow fluorescent protein. Scale bar = 100 μm