Fig. 5
From: Rust effector PNPi interacting with wheat TaPR1a attenuates plant defense response
The NPR1-mediated acquired resistance to Magnaporthe oryzae isolate P131 was suppressed in the transgenic wheat line SP-PNPi-OE. a Expression levels of the SP(TaPR1a)-PNPi-cMYC transgene in the transgenic wheat line SP-PNPi-OE were determined by RT-qPCR. b P. syringae DC3000 was employed to induce the acquired resistance in the region adjacent to the infiltration area. c The M. oryzae isolate P131 was inoculated in the adjacent region as a secondary pathogen to evaluate the degree of the acquired resistance. The numbers below the images of the leaves represent the average lesion size caused by M. oryzae. d The expression levels of TaPR1a, TaPR2, and TaPR4b in transgenic wheat line SP-PNPi-OE and wild-type plants during acquired resistance were determined by RT-qPCR. The whole experiment was repeated twice using two independent transgenic lines of SP-PNPi-OE. Statistical analyses are presented in Additional file 1: Table S5