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Fig. 2 | Phytopathology Research

Fig. 2

From: Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clones

Fig. 2

One-step verification of complete Potyvirus clone sequence by Illumina sequencing. a The sequence of the 13.6-kb pLX-TVMV vector was verified using the Illumina platform and de novo read assembly; the read coverage (log10), vector backbone and TVMV sequences are indicated. b The sequencing depth (read number, orange) and error rate (gray) of the TVMV genome assembled are plotted. c Dot plots show significant DNA alignments between pLX-TVMV (this study, GenBank: MW027845) and pLX-B2 (GenBank: KY825137) or a TVMV reference genome (GenBank: X04083); axis ticks indicate 1-kb intervals

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Phytopathology Research

ISSN: 2524-4167