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Fig. 3 | Phytopathology Research

Fig. 3

From: Home-made enzymatic premix and Illumina sequencing allow for one-step Gibson assembly and verification of virus infectious clones

Fig. 3

Agrobacterium-mediated delivery to plants of an intron-free, pLX-based cDNA clone of tobacco vein mottling virus (TVMV). pLX-TVMV was transformed into Agrobacterium and delivered to Nicotiana spp. by agro-infection; data were collected after 18 d. a Symptoms of the pLX-TVMV-treated N. clevelandii plants; untreated plants are shown as a control (Healthy). b Viral accumulation was assessed by anti-TVMV coat protein (CP) immunoblotting of upper uninoculated N. clevelandii leaves; H, untreated control; Ponceau red-stained blot is shown as a loading control. c Transmission electron micrograph of particles observed in infected N. clevelandii material; scale bar, 200 nm. d Symptoms of the pLX-TVMV-treated N. benthamiana plants; healthy, an untreated control. e Transmission electron micrograph of particles observed in infected N. benthamiana material; scale bar, 200 nm

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