Reagent | Provider | Identifier |
---|---|---|
Tris(hydroxymethyl)aminomethane (Tris base) | Sigma-Aldrich | T1503 |
Poly(ethylene glycol) MW ~ 8000 (PEG-8000) | MP Biomedicals | 195445 |
MgCl2 | Sigma-Aldrich | 442611 |
Dithiothreitol (DTT) | Promega | V3151 |
dNTP mix | Thermo Fisher Scientific | R1121 |
β-Nicotinamide adenine dinucleotide (NAD+) | New England Biolabs | B9007S |
T5 exonuclease | New England Biolabs | M0663S |
Phusion® High-Fidelity DNA polymerase | New England Biolabs | M0530S |
Taq DNA ligase | New England Biolabs | M0208S |
Nuclease-free distilled H2O (n.f. dH2O)a | – | – |