Table 3 GA enzymatic premix (2×, 125 μL)
Reagent | Stock concentration | Volume | Final concentration |
|---|---|---|---|
GA reaction buffer | 3.33× | 75.8 μL | 2× |
T5 exonucleasea | 0.1 U/μL | 10.0 μL | 0.008 U/μL |
Phusion® High-Fidelity DNA polymerase | 2 U/μL | 3.1 μL | 0.05 U/μL |
Taq DNA ligase | 40 U/μL | 25.0 μL | 8 U/μL |
n.f. dH2O | – | 11.1 μL | – |
Total volume | – | 125.0 μL | – |