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Fig. 5 | Phytopathology Research

Fig. 5

From: The bZIP transcription factor PsBZP32 is involved in cyst germination, oxidative stress response, and pathogenicity of Phytophthora sojae

Fig. 5

Subcellular localization and phosphorylation status of PsBZP32 are regulated by oxidative stress. a Localization of PsBZP32::GFP under the native promoter of PsBZP32. “H2O2” indicates that the mycelium was treated with 5 mM H2O2 for 10 min. Nuclei were stained with DAPI for 5 min. Bar, 20 μm. b Statistical analysis of the results in (a) using the two-tailed t-test (*, P < 0.01). c and d Cytoplasmic and nuclear proteins extracted from mycelia treated with or without 5 mM H2O2 for 5 min were subjected to SDS-PAGE without (c) or with Phos-tag (d). A polyclonal antibody against prokaryotically expressed PsBZP32 was used to detect the level of phosphorylated PsBZP32 in western blot assay. CT, cytoplasmic protein; NU, nuclear protein. Red arrows indicate the bands representing phosphorylated PsBZP32

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