Fig. 1

Expression pattern, multiple sequence alignment and phylogenetic analysis of NbERF173. a Expression levels of NbERF173 in N. benthamiana in response to P. parasitica infection. N. benthamiana leaves infected with P. parasitica at the indicated time points were used for RNA extraction. The expression level of NbERF173 was analyzed by qRT-PCR using EF1α gene as a control. The error bars represent the standard deviation. Data are the mean ± SE from three independent experiments. b Phylogenetic tree of the NbERF173 orthologous. The close orthologous from N. benthamiana and other plants, including NtERF071 (N. tabacum, NP_001311590.1), AtERF (Arabidopsis thaliana, NP_188299.1), CaERF071 (Capsicum annuum, NP_001311812) and SlERF2 (Solanum lycopersicum, AAO34704.1) were used. c Amino acid comparison of the NbERF173 orthologous from various species. An amino acid identity of 100% is indicated by the black background; amino acids with conservation exceeding 50% are in black on a gray background; similar amino acids are in white on a gray background. AP2 domain was underlined in red (135 to 187). The asterisks represent the nuclear export signals (203 to 212)