Fig. 2

Overexpression of NbERF173 boosts resistance of N. benthamiana to P. parasitica. a Subcellular localization of NbERF173. The constructs, pBINPLUS:NbERF173 and pBINPLUS:GFP, were transiently expressed in N. benthamiana leaves. The photos were taken using a confocal microscope. The excitation wavelength used was 488 nm for GFP and 405 nm for DAPI. The scale bar indicates 50 μm. b Representative photographs of N. benthamiana leaves under infection. A. tumefaciens-mediated transient expression of CK (right) and NbERF173 (left) fusion proteins in N. benthamiana leaves. The infiltrated leaves (after 48 h) were inoculated with P. parasitica zoospores, and photographed at 36 hpi and 48 hpi, respectively. c Lesion diameters (mm) of N. benthamiana leaves. The data was calculated from three independent biological replicates using at least eight leaves each (** P < 0.01 compared with GFP; Dunnett’s test)