Fig. 6
From: Genome-wide identification and molecular evolution analysis of BPA genes in green plants
Physical interactions between Arabidopsis BPAs. a Physical interactions between Arabidopsis BPAs revealed by Y2H assay. Arabidopsis BPA genes were cloned into the bait vector pGBKT7 (BD) and the prey vector pGADT7 (AD). The combination of BD-53 and AD-T was used as the positive control. BD-Lam and AD-T combination was used as the negative control. Yeast transformants were grown on selective medium (SD) lacking Tryptophan (T) and Leucine (L) and selected on SD lacking Tryptophan (T), Leucine (L), Histidine (H) and Adenine (A). Plates were photographed 3 days after inoculation. b Summary of the interactions. “+” represents interaction. “-” represents no interaction