Fig. 5

The impact of deleting sboA or bacG on colonization ability of the B. subtilis 9407 strain. a, b Biofilm formation assays. Biofilm assays were detected in Msgg plate incubated at 28 °C for 96 h. c, d Swarming ability assays. Swarming assays were detected in LA plate (containing 0.7% agar) incubated at 37 °C for 6 h. After drying the water, the plates were incubated overnight at 37 °C. e Colonization on the melon roots (R) and leaves (L) of B. subtilis 9407 and mutant strains. To facilitate the screening, all these strains were transformed into a plasmid pC-1 with a chloramphenicol resistance gene. The y-axis was log₁₀CFU and the x-axis was the number of days. The statistical analysis was performed using GraphPad Prism 7 software by one-way ANOVA with the Dunnett test (P < 0.05). The variation was recorded as mean ± SE (****: P < 0.0001, ***: P < 0.001, **: P < 0.01, *: P < 0.05)