Fig. 1

CSEP0139 and CSEP0182 contain a functional signal peptide. a Schematic representation of CSEP0139 and CSEP0182, the N-terminal signal peptide (SP) was predicted by Signal 4.0 (http://www.cbs.dtu.dk/services/SignalP), highlighted in red. Shades of blue indicate F/YxC motif. b Yeast invertase secretion assay for the verification of SP. The SP sequences of CSEP0139, CSEP0182, and P. sojae PsAvr1b and the N-terminal sequence of M. oryzae Mg87 were individually fused to yeast invertase lacking its own SP in the pSUC2 vector. CMD-W (minus Trp) media were used to select positive transformants of yeast mutant strain YTK12 carrying the pSUC2 vector. Yeast growth on YPRAA media indicates secretion of invertase. The pictures were taken at 3 days after plating