Fig. 2

CSEP0139 and CSEP0182 inhibit BAX- and NtMEK2^DD-induced cell death in N. benthamiana. a Suppression of BAX-triggered cell death by CSEP0139 and CSEP0182 in N. benthamiana. b Suppression of NtMEK2^DD-induced cell death by CSEP0139 and CSEP0182 in N. benthamiana. The Oomycete effector Avh328 was used as a positive control, and CSEP0340 as a negative control. Expression of CSEPs, BAX and NtMEK2^DD was done by agroinfiltration in different but overlapping areas in leaves of N. benthamiana, with 12 h time lapse between the agroinfiltration of the effector and the cell death inducer. Cell death was visualized by trypan blue staining, and photographs were taken at 5 days after infiltration. Protein accumulation of CSEP fusions were detected at 2 days after infiltration by Western blotting