Table 4 Mutations in the coding regions of AaHK1 from Alternaria alternata ProHR and ProS isolates
Isolate | Origin | Nucleotide variation in coding region | Mutation in amino acid sequence | Structural domain |
|---|---|---|---|---|
C10, C28 | Cabbage /ProS | WT (N) | N | N |
B16, B21 | Broccoli /ProS | WT (N) | N | N |
C5 | Cabbage /ProHR | CCC to GCC at nt280 | P94A | Between N-terminus and HAMP1 |
C13 | Cabbage /ProHR | 552-bp deletion between nt791 – nt1344 | A deletion of 184-AA residues | Between HAMP 1 and HAMP 3 |
C40 | Cabbage /ProHR | 8-bp insertion between nt3294 – nt3295 | Frameshift mutation leading to premature termination at the codon1204 | Rec |
B30 | Broccoli /ProHR | GTG to CTG at nt1834 | V612L | HAMP5 |
B88, B108 | Broccoli /ProHR | GAG to AAG at nt2122 | E708K | HAMP6 |
B35 | Broccoli /ProHR | CAG to TAG at nt2770 | Q924STOP | HATPase_c |
B46 | Broccoli /ProHR | 381-bp deletion between nt1707 – nt2089 | A deletion of 127-AA residues | Between HAMP4 and HAMP6 |
B53 | Broccoli /ProHR | 2-bp deletion between nt3249 – nt3252 | Frameshift mutation leading to premature termination at the codon1104 | Between HATPase_c and Rec |
B102 | Broccoli /ProHR | N | N | N |