Fig. 5

The peroxisome localization of ZmCATs is required for viral replication of maize chlorotic mottle virus (MCMV). a, b Subcellular localization of ZmCATs in maize protoplasts in the presence of MCMV in vitro transcripts (a) and in MCMV-infected leaves (b). The green/red fluorescence was observed at 24 hpt (a) and 36 hpb (b), respectively. Bars, 10 μm. c, d GFP-ZmCAT1 co-expressed with DsRed-tagged peroxisome marker SKL in MCMV-infected maize protoplasts (c) and MCMV-inoculated maize leaves (d). The green/red fluorescence was observed at 24 hpt (c) and 36 hpb (d), respectively. Bars, 10 μm. e Protoplasts were inoculated with MCMV in vitro transcripts and incubated at 25 °C for 18 h in the presence of 20 mM 3-AT (3-amino-1, 2, 4-triazole) or 10 μM MV (methyl viologen). The accumulation levels of MCMV genomic RNA were determined by Northern blot and visualized with the IMAGE J software. The numbers shown below the gel indicate the ratio of MCMV genomic RNA (top band) accumulation level in 3-AT/MV-treated maize protoplasts to that in the H₂O-treated control maize protoplasts (presented as 100). Relative genomic RNA accumulation was calculated from maize protoplast Northern blot data for three separate inoculation experiments and represents mean ± standard error