Fig. 2

PCR amplification of the genomic region containing spacer 1029 in different strains of E. amylovora using primers C1f04 and C1r09. Strains bearing two copies of spacer 1029 (A-derived genotype, blue) yielded a 276 bp amplicon, while those bearing a single copy (D-derived genotype, green) resulted in a shorter band at 215 bp. Strain Tk119, which displays an extensive deletion at the 5′ end of the array that includes both primer annealing sites, did not yield (as expected) any amplification. One of the four additional Turkish isolates with unknown genotypes tested (Tk3) revealed a D-derived genotype, while two others (Tk11 and Tk86) could be assigned to the A-derived genotype. A fourth isolate (Tk2) displayed a larger band on gel that indicates the generation of a longer PCR product. Sanger sequencing of the purified 337-bp amplicon revealed the presence of a third copy of spacer 1029, a result that is compatible with the estimated size of the band on gel. CFBP 3098 and CFBP 1430 were used as positive controls for D- and A-derived genotypes, respectively. PCR-grade water was used as the negative non-template control (NTC)