Fig. 1

Symptoms and detection of PVX in different potato cultivars. a RT-PCR analysis of the presence of PVX in potato plantlets using primer pair pvx-tgb2-f and PVXcpRT-R. M, DL2000 DNA marker (Vazyme); P, PVX-infected Nicotiana benthamiana leaf tissues (positive control); N, healthy N. benthamiana leaf tissues (negative control). Potato cultivars were arranged in the same order as in Table 1. b PVX symptoms on plantlets of Netherlands-15 and Kexin-18 at 12 dpi. c RT-PCR analysis of the presence of PVX in the systemic leaves of potato plantlets using primers PVX5875-F and PVXcpRT-R at 15 dpi. Lanes 1, 3, 5, 7, 9 and 11 are healthy Kexin-1, Atlantic, Eugene, Waiyin-2, Longshu-3 and Waiyin-1, respectively; lanes 2, 4, 6, 8, 10 and 12 are PVX-infected Kexin-1, Atlantic, Eugene, Waiyin-2, Longshu-3 and Waiyin-1, respectively. d Detection of PVX genome in Waiyin-1 and Kexin-1 using primers PVX5875-F and PVXcpRT-R at 9, 12 and 15 dpi. H, Healthy Kexin-1; P, positive control (PVX-infected N. benthamiana leaf tissues); lanes 1, 2 and 3 are systemic leaves of Kexin-1 agroinfiltrated with pGR107 at 9, 12 and 15 dpi, respectively; lanes 4, 5 and 6 are systemic leaves of Waiyin-1 agroinfiltrated with pGR107 at 9, 12 and 15 dpi, respectively