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Fig. 3 | Phytopathology Research

Fig. 3

From: Three highly sensitive monoclonal antibody-based serological assays for the detection of tomato mottle mosaic virus

Fig. 3

Specificity and sensitivity analyses of the six MAbs using ACP-ELISA. Extracts were prepared from ToMMV-, ToMV-, TMV-, CMV- or CGMMVV-infected plant tissues, with the extract from uninfected tomato samples as a negative control. The extracts were diluted 1:30 (w/v) prior to ACP-ELISA (a) or serially diluted in the coating buffer (1:10 to 1:163,840 w/v) prior to ACP-ELISA (b). The optical density (OD) at 405 nm was read at 30 min post adding the substrate and expressed as mean ± standard deviation (SD) of three biological replications. ***P ≤ 0.01

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