Fig. 4
Specificity and sensitivity analyses of the developed dot-ELISAs and conventional RT-PCR. a Specificity evaluation of the developed dot-ELISAs. Extracts were prepared from ToMMV-, ToMV-, TMV-, CMV- or CGMMV-infected plant tissues. The extract from uninfected tomato samples was used as a negative control. For each MAb, two dots on the same membrane were two biological repeats. The light to dark purple color indicates a positive reaction. b Sensitivity evaluation of the developed dot-ELISAs. The extracts from ToMMV-infected and uninfected tomato plants were serially diluted from 1:20 to 1:81,920 (w/v) in PBS (pH 7.4). The light to dark purple color indicates a positive reaction. c Specificity and sensitivity evaluations of the one-step RT-PCR method. Total RNA (30 µL) purified from 100 mg of ToMMV-infected tomato plant tissues was serially two-fold diluted from 1:4 to 1:2048 (w/v) in DNase/RNase free ddH2O, and 1 μL of each RNA dilution was used to determine the sensitivity