Fig. 2

VdEIX3, INF1, RXLR25 and PsAvh163 induce SGT-dependent HR, while INF1, RXLR25, PsAvh163, PsAvh241 and PsCRN63 cause HSP90-dependent HR. a Phenotypes of eight oomycete effectors in GUS-, SGT1- and HSP90-silenced leaves. INF1, PoNLP5, PoNLP7, PcRXLR25, PsAvh105, PsAvh163, PsAvh241 and PsCRN63 were transiently expressed individually through Agrobacterium infiltration in gene-silenced leaves of N. benthamiana. Agrobacterium was infiltrated at an OD600 of 0.5 and the plants were imaged at 6 dpi. Consistent results were observed from three independent biological replicates. Numbers represent the number of leaves showing cell death out of the number of leaves analyzed. b Western blot detection of the effectors that trigger SGT1 or HSP90-dependent cell death. The accumulation of VdEIX3, INF1, PcRXLR25, PsAvh163, PsAvh241 and PsCRN63 in GUS-, SGT1- and HSP90-silenced plants. GFP was used as a test control. Total proteins were extracted from the leaves at 36 hpi, and protein accumulation was determined using anti-GFP or anti-HA antibody. In western blots, total protein loading was confirmed by Ponceau S staining