Fig. 5
From: Different requirement of immunity pathway components by oomycete effectors-induced cell death
Silencing NbNRC2, NbNRC3 and NbNRC4 simultaneously attenuates the cell death triggered by PcRXLR25 and PsAvh163 in N. benthamiana. a Silencing efficiency of NbNRC2, NbNRC3 and NbNRC4. Values represented average ± SE (n = 3); **, P < 0.01 compared with the GUS; Student’s t-test. b Transient expression of GFP, PcRXLR25, PsAvh163 and PsAvh105 in GUS- and NRC2/3/4-silenced plants. GFP was used as a negative control. Consistent results were observed from three independent biological experiments. Numbers represent the number of leaves showing cell death out of the number of leaves analyzed. c The accumulation of GFP, PcRxLR25, PsAvh163 and PsAvh105 in GUS- and NRC2/3/4-silenced plants. GFP was used as a negative control. Total protein loading was confirmed by Ponceau S staining