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Fig. 5 | Phytopathology Research

Fig. 5

From: The Pseudomonas syringae effector AvrPtoB targets abscisic acid signaling pathway to promote its virulence in Arabidopsis

Fig. 5

AvrPtoB increases ABA sensitivity in Arabidopsis. a Ecotopic expression of AvrPtoB increases the sensitivity to ABA in plants. The Arabidopsis seedlings were grown on ½ MS containing 0.1 μM ABA or 50 μM estradiol or both for 7 days. Scale bar = 2.5 mm. b Seed germination greening ratio in (a). Error bars represent means ± SD (Two-way ANOVA, P < 0.01, n = 3). c AvrPtoB protein levels in pEst:AvrPtoB transgenic plants. The AvrPtoB proteins were shown in two independent AvrPtoB transgenic lines after 50 μM estradiol treatment (a). d and e AvrPtoB induces ABA regulatory gene expression. The two-week-old transgenic plants were sprayed with 50 μM estradiol. The plant leaves were sampled at 12 hpi for RT-qPCR assays. Error bars represent means ± SD (Two-way ANOVA, P < 0.0001, n = 3)

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Phytopathology Research

ISSN: 2524-4167