Fig. 5

Symptoms and accumulation of SRBSDV in roc1 mutant lines and WT rice plants. a The symptoms caused by SRBSDV infection in WT and roc1 plants. The plants were inoculated with viruliferous white-backed planthopper, and the photographs were taken at 20 dpi. Bars = 10 cm. b The average root length of WT and roc1 rice plants. Inoculation experiments were repeated 3 times with 10 plants each. Values represent the mean root length ± SD from three independent experiments. One-way ANOVA followed by a multiple-comparisons Tukey’s test was used for the analyses (*P < 0.05). c RT-qPCR analysis of SRBSDV RNA accumulation in WT and roc1 plants. Total RNAs were extracted from SRBSDV-infected leaves at 20 dpi. The values shown are the means ± SD (n = 3). Student’s t test was used for the analyses (*P < 0.05). d Western blot analysis of SRBSDV encoded P10 protein accumulation in WT and roc1 plants. Total proteins were extracted from SRBSDV-infected leaves at 20 dpi and were detected by P10-specific antibodies