Fig. 1

The recombinant virus vector pSVBV-P1-MCS could induce systemic infection in strawberry plants. a Schematic representation of the construction of pSVBV and pSVBV-P1-MCS vector. A total of 1.25 copies of the full-length SVBV genome were inserted into pCB301 to construct the infectious clone pSVBV. The MCS sequence was CACGTGACGGGATCC, with the underlined part representing the recognition sequence of restriction endonucleases PmlI and BamHI. Fragment P1-MCS was cloned into the plasmid pSVBV via the EcoNI/DraIII to generate pSVBV-P1-MCS, which has a MCS downstream of the P1 gene; b Light-green vein banding symptoms were observed in systemic leaves of strawberry plants agroinfiltrated with pSVBV or pSVBV-P1-MCS. Strawberry plants agroinfiltrated with pCB301 were used as negative controls; c Accumulation of SVBV in pCB301-, pSVBV-, or pSVBV-P1-MCS-agroinfiltrated strawberry plants determined by Southern blot analysis. Blot was probed with the CP gene sequence of SVBV. The lower panel represents an ethidium bromide-stained gel of DNA samples as a loading control