Fig. 2

Expression of GFP in strawberry plants inoculated with the pSVBV-P1-GFP vector. a Schematic representation of the overexpression vector pSVBV-P1-GFP. The GFP gene was cloned into pSVBV-P1-MCS via the PmlI-BamHI sites. b Detection of fluorescence signals in the leaves of strawberry plants inoculated with pSVBV-P1-MCS or pSVBV-P1-GFP. The back of the strawberry leaves was illuminated under UV-B light. c Western blot analysis of the leaf samples shown in (b) using antibodies for SVBV P4 and GFP. Coomassie brilliant blue (CBB)-staining of the RuBisCO large subunit (rbcL) was used as a loading control