Fig. 3
From: Strawberry vein banding virus-based vector for transient overexpression in strawberry plants
The recombinant virus vector pSVBV-P4-MCS could induce systemic infection in strawberry plants. a Schematic representation of the pSVBV-P4-MCS vector. The MCS sequence was CACGTGACGGGATCC, with the underlined part representing the recognition sequence of restriction endonucleases PmlI and BamHI. The fragment P4-MCS was cloned into the plasmid pSVBV via the DraIII/Bsp1407I sites to generate pSVBV-P4-MCS, which has a MCS downstream of the P4 gene; b Light-green vein banding symptoms in strawberry plants agroinfiltrated with pSVBV or pSVBV-P4-MCS. Strawberry plants agroinfiltrated with pCB301 were used as negative controls; c Accumulation of SVBV in pCB301-, pSVBV-, or pSVBV-P4-MCS-agroinfiltrated strawberry plants by Southern blot analysis. SVBV DNA was detected using a digoxigenin-labeled probe specific for the SVBV CP gene. The lower panel represents an ethidium bromide-stained gel of DNA samples as a loading control