Fig. 4
From: Strawberry vein banding virus-based vector for transient overexpression in strawberry plants
Expression of GFP in strawberry plants using the pSVBV-P4-GFP vector. a Schematic representation of the overexpression vector pSVBV-P4-GFP. The GFP gene was cloned into pSVBV-P4-MCS via the PmlI-BamHI sites. b Detection of fluorescence signals in the leaves of strawberry plants inoculated with pSVBV-P4-MCS or pSVBV-P4-GFP. Backs of the strawberry leaves were illuminated under UV-B light. c Western blot analysis of leaf samples shown in (b) using antibodies for SVBV P4 and GFP. Coomassie brilliant blue (CBB)-staining of the RuBisCO large subunit (rbcL) was used as a loading control