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Fig. 5 | Phytopathology Research

Fig. 5

From: Oryzae pathotype of Magnaporthe oryzae can cause typical blast disease symptoms on both leaves and spikes of wheat under a growth room condition

Fig. 5

PCR detection of MoT and MoO pathotypes. The gel images of PCR products amplified by Pot2 (a), MoT3 (b) and MoT6099 primers (c) to confirm the M. oryzae pathotype. The gDNA used in this study was isolated from mycelia of the respective fungus. Lane 1, DNA ladder; Lanes 2 and 3 represent DNA isolated from MoT strains BTJP4-5 and BTMaU(10b), respectively, which were collected from naturally blast infected wheat field; Lanes 3 and 4 denote DNA isolated from MoO isolates RB13b and RBTa1849-2, respectively, which were collected from naturally blast infected rice field; Lanes 5 and 6 represent DNA reisolated from the symptomatic tissues of wheat spikes artificially inoculated with the MoO strains RB13b and RBTa1849-2, respectively

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Phytopathology Research

ISSN: 2524-4167