Fig. 7
Viral accumulation and induction of immune responses by SMVNB and SMVGZL in soybean and N. benthamiana. a Symptoms on systemic (non-inoculated) leaves of Dongnong 51 plants at 15 dpi. b RNA levels of SMVNB and SMVGZL in infected plants as determined by RT-qPCR. Data are shown as mean ± SD of three biological replicates. **P < 0.01, Student’s t tests. c Immunoblots showing levels of SMV coat protein (CP) levels in plants mock-inoculated or inoculated with SMVNB/SMVGZL. Coomassie brilliant blue (CBB) staining of the same extracts shows equal loading. d Transcript levels of GmNPR1 and GmNPR2 in soybean plants infected with SMVNB or SMVGZL as determined by RT-qPCR. Average SMVGZL value equal to 100. e Levels of endogenous salicylic acid (SA) in mock and SMV-infected soybean plants at 15 dpi. Data are shown as mean ± SD of three biological replicates. **P < 0.01, Student’s t tests. f Transcript levels of NbPR1, NbNPR1 and NbSABP2 in N. benthamiana plants transiently expressing P1NB or P1GZL as determined by RT-qPCR. Average P1GZL value equal to 100. The numbers above the error bars indicate the fold change of gene expression compared with P1GZL in N. benthamiana (WT) and transgenic N. benthamiana NahG plants. Statistically significant differences indicated by different lowercase letters are analyzed by Student’s t tests (P < 0.05). Data are shown as mean ± SD of three biological replicates