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Fig. 3 | Phytopathology Research

Fig. 3

From: Autophagy-related protein UvAtg14 contributes to mycelial growth, asexual reproduction, virulence and cell stress response in rice false smut fungus Ustilaginoidea virens

Fig. 3

UvAtg14 is essential for autophagy in U. virens. a Subcellular localization of GFP-UvAtg8 in wild-type strain JT209, UvATG14 deletion mutant and complementary strain under normal (PSB) and nitrogen-starvation (SD-N) conditions. Vacuoles were stained with CMAC (7-amino-4-chloromethylcoumarin). b Detection of proteolysis of GFP-UvAtg8 by Western blot. A main band of total protein stained by Ponceau S was used as a control. c The percentage of free to total GFP. Three replicates were performed and values are expressed as mean ± standard deviation. *, the significant difference at the 0.05 level (t-test) . NS, no significant difference

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