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Fig. 4 | Phytopathology Research

Fig. 4

From: Autophagy-related protein UvAtg14 contributes to mycelial growth, asexual reproduction, virulence and cell stress response in rice false smut fungus Ustilaginoidea virens

Fig. 4

UvAtg14 contributes to mycelial growth, asexual reproduction, and pathogenicity in U. virens. a Colonies of wild-type strain JT209, UvATG14 deletion mutant ΔUvATG14-130 and complementary strain cUvATG14-130–1 cultured on PSA, SD, and SD-N media at 28ºC in the dark for 20 days. b Rice panicles inoculated with JT209, ΔUvATG14-130, and cUvATG14-130–1 for 30 days. c Rice false smut balls generated by JT209, ΔUvATG14-130, and cUvATG14-130–1. d Column diagram showing colony diameter of JT209, UvATG14 deletion mutants (ΔUvATG14-130, ΔUvATG14-516, and ΔUvATG14-517) and complementary strains (cUvATG14-130–1, cUvATG14-130–2). e Conidiation capacity of U. virens strains in d incubated in 1%YT broth at 28ºC, 160 r/min, in the dark for 5 days. f The number of false smut balls per panicle artificially inoculated with U. virens strains in d. Three replicates were performed and values are expressed as mean ± standard deviation. ** and *, significant difference at the 0.01 level and 0.05 level (t-test) compared to wild-type strain JT209, respectively

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