Fig. 4
Transcription of the hca gene cluster. a RT-PCR analysis of the hca gene cluster. Lanes 1–4 indicate agarose gel analysis of PCR products generated with the indicated primers and using the following templates: XC1 genomic DNA(1); total RNAs from 4-HCA-treated XC1(2); cDNA from total RNAs of 4-HCA-treated XC1(3); ddH2O(4). b Colonies of four promoter-driven strains on NYG agar media supplemented with 50 μg/mL x-Gluc in the absence or presence of 50 μM 4-HCA and GUS activities of four promoter-driven strains in NYG liquid medium in the presence of 50 μM 4-HCA. c Colonies of strain XC1::Phca-gusA grown on NYG agar medium supplemented with 50 μg/mL of x-Gluc and different HCAs and GUS activities of strain XC1::Phca-gusA grown on NYG medium with various HCAs added (0–200 μM). Three independent experiments were conducted and average values with standard deviations are shown. Values in treatments with HCAs added which were significantly different from the value in the absence of HCAs are indicated by one (P < 0.05), two (P < 0.01) or three (P < 0.001) asterisks