Fig. 6From: The phytopathogen Xanthomonas campestris scavenges hydroxycinnamic acids in planta via the hca cluster to increase virulence on its host plantElectrophoretic mobility shift assays (EMSA) for the identification of the HcaR binding site in the promoter region of the hca operon. a SDS-PAGE analysis of the purified HcaR protein. b EMSA was performed using a 183-bp Cy5-labeled DNA probe, Phca and 0.05–5.0 nM HcaR protein. 2000 nM unlabeled Phca was added as a cold probe. c DNase I footprint sequencing was performed using purified HcaR protein and a 335-bp DNA probe Phca. d The HcaR binding site in the promoter of hcaR. The -10/-35 sequences and the Shine-Dalgarno sequence are underlined and labeled with -10, -35 and SD, respectively. The sequence of the protection region is shown in red. e EMSA of the mutant probe Cy5-Phca(C) in the presence of HcaR proteinBack to article page