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Fig. 6 | Phytopathology Research

Fig. 6

From: The phytopathogen Xanthomonas campestris scavenges hydroxycinnamic acids in planta via the hca cluster to increase virulence on its host plant

Fig. 6

Electrophoretic mobility shift assays (EMSA) for the identification of the HcaR binding site in the promoter region of the hca operon. a SDS-PAGE analysis of the purified HcaR protein. b EMSA was performed using a 183-bp Cy5-labeled DNA probe, Phca and 0.05–5.0 nM HcaR protein. 2000 nM unlabeled Phca was added as a cold probe. c DNase I footprint sequencing was performed using purified HcaR protein and a 335-bp DNA probe Phca. d The HcaR binding site in the promoter of hcaR. The -10/-35 sequences and the Shine-Dalgarno sequence are underlined and labeled with -10, -35 and SD, respectively. The sequence of the protection region is shown in red. e EMSA of the mutant probe Cy5-Phca(C) in the presence of HcaR protein

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