Fig. 3
From: Identification of the blast resistance genes in three elite restorer lines of hybrid rice
Expression profiling of rice blast R genes in SH548, SH882, and WSSM. a–c RT-PCR analysis of the cloned rice blast R genes in SH548, SH882, and WSSM, respectively. Three-week-old seedlings of SH548, SH882, and WSSM were spray-inoculated with mixed spores (3 × 105 spores/mL) of CRB strains including CRB1, CRB4, CRB9, CRB10, CRB13, CRB14, CRB15, CRB18, and CRB21. The leaves of each line were collected at 0, 12, 24, and 48 hpi. OsEF-1α was used as an internal control. d–f RT-qPCR analysis of the rice blast R genes, including Pi2 in SH548, Pikm (Pikm1 and Pikm2) in SH882, and Pi9 in WSSM. Leaf samples were collected at 0, 12, 24, and 48 hpi with Guy11 (4 × 105 spores/mL). Different letters above the bars indicate significant differences (P < 0.05, one-way ANOVA, post hoc Tukey HSD analysis). Error bars indicate SD (n = 3). All the experiments were repeated two times with the same results