Fig. 6

Biomasses of Simplicillium aogashimaense and Blumeria graminis f. sp. tritici (Bgt) on wheat leaves at different time points. The DNA concentration standard curves of wheat (Triticum aestivum, Ta) (a), S. aogashimaense (Sa) (b) and Bgt (c) were used to calculate the biomasses of S. aogashimaense (d) and Bgt (e). The standard curves derived from serially diluted DNA samples were obtained using the primers of elongation factors of Ta, Sa and Bgt. Wheat leaves were inoculated with Bgt for 6 days, followed by inoculation with a suspension of Sa conidia for biomass calculation. Data are represented as mean ± standard deviation from three independent biological experiments. The means were compared using one-way analysis of variance, followed by Tukey’s post-hoc test. Different letters indicate significant differences (P < 0.05)