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Table 3 Amino acid substitution in Cyt b and sensitivity of Podosphaera xanthii isolates or mutants to ZJ0712 and four other fungicides

From: Evaluation of the risk of development of resistance to QoI fungicide ZJ0712 in Podosphaera xanthii under greenhouse conditions

Isolatea Substitutionb EC50 (μg/mL)
ZJ0712 Azoxystrobin Enostrobilurin Chlorothalonil Triadimefon
N1 WTc 0.011 0.012 0.048 0.232 0.179
N2 WT 0.012 0.021 0.039 0.215 0.237
N3 WT 0.013 0.014 0.071 0.250 0.214
N4 WT 0.013 0.028 0.054 0.309 0.225
BH17 WT 0.008 0.010 0.019 0.122 0.200
SR1 G143A 1.267 1.287 1.865 1.967 0.254
BR14 G143A 1.575 1.880 1.966 1.893 0.174
NR1 I161M 1.704 1.683 2.303 2.164 0.148
NR2 A113G/G143A 1.725 1.605 1.510 2.175 0.182
NR3 A113G/G143A/I161M 3.201 5.321 1.592 2.164 0.187
NR4 A113G/G143A 3.260 3.531 1.769 1.997 0.194
NR5 A113G/G143A 2.322 2.832 1.966 3.087 0.182
NR6 I161M 2.718 2.832 1.402 1.884 0.179
NR7 G143A 2.007 5.321 1.358 2.725 0.189
  1. aN1–N4 and BH17 are the wild-type isolates; SR1 and BR14 are ZJ0712-resistant mutants obtained by fungicide adaption in our previous study; NR1–NR7 are ZJ0712-resistant isolates collected from the greenhouse in this study
  2. bAmino acid substitution genotype based on DNA sequencing of the cyt b gene
  3. cWT, wild-type