Phytopathology Research

Table 3 Amino acid substitution in Cyt b and sensitivity of Podosphaera xanthii isolates or mutants to ZJ0712 and four other fungicides

From: Evaluation of the risk of development of resistance to QoI fungicide ZJ0712 in Podosphaera xanthii under greenhouse conditions

Isolate^a	Substitution^b	EC₅₀ (μg/mL)
Isolate^a	Substitution^b	ZJ0712	Azoxystrobin	Enostrobilurin	Chlorothalonil	Triadimefon
N1	WT^c	0.011	0.012	0.048	0.232	0.179
N2	WT	0.012	0.021	0.039	0.215	0.237
N3	WT	0.013	0.014	0.071	0.250	0.214
N4	WT	0.013	0.028	0.054	0.309	0.225
BH17	WT	0.008	0.010	0.019	0.122	0.200
SR1	G143A	1.267	1.287	1.865	1.967	0.254
BR14	G143A	1.575	1.880	1.966	1.893	0.174
NR1	I161M	1.704	1.683	2.303	2.164	0.148
NR2	A113G/G143A	1.725	1.605	1.510	2.175	0.182
NR3	A113G/G143A/I161M	3.201	5.321	1.592	2.164	0.187
NR4	A113G/G143A	3.260	3.531	1.769	1.997	0.194
NR5	A113G/G143A	2.322	2.832	1.966	3.087	0.182
NR6	I161M	2.718	2.832	1.402	1.884	0.179
NR7	G143A	2.007	5.321	1.358	2.725	0.189

^aN1–N4 and BH17 are the wild-type isolates; SR1 and BR14 are ZJ0712-resistant mutants obtained by fungicide adaption in our previous study; NR1–NR7 are ZJ0712-resistant isolates collected from the greenhouse in this study
^bAmino acid substitution genotype based on DNA sequencing of the cyt b gene
^cWT, wild-type

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