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Fig. 4 | Phytopathology Research

Fig. 4

From: Targeted isolation of biocontrol agents from plants through phytopathogen co-culture and pathogen enrichment

Fig. 4

Targeted isolation of antibacterial and antifungal Bacillus velezensis ZK-3 from rice seeds by co-enrichment of the pathogen Xanthomonas oryzae and the fungal pathogen Magnaporthe oryzae. a Antibacterial activity of live cells (A–C) or cell-free supernatant (D–F) of ZK-3 against three selected pathogens. A and D, LB plate pre-inoculated with Xanthomonas oryzae PXO99; B and E, LB plate pre-inoculated with Xanthomonas campestris 8004; C and F, LB plate pre-inoculated with Acidovorax citrulli xjl12. b, c Antifungal test of the live cells (b) or cell-free supernatant (c) of ZK-3 against four selected fungal pathogens. G, H, I and J, PDA plates pre-inoculated with Magnaporthe oryzae, Clarireedia paspali, Botrytis cinerea and Valsa pyri, respectively. 1#, ZK-3; 2# and 3#, positive controls (2#, Lysobacter enzymogenes OH11; 3#, Bacillus subtilis NCD-2) with known antifungal activity; 4#, negative control, E. coli Top10. d Phylogenomic tree based on the genome sequences of strain ZK-1 in TYGS (https://tygs.dsmz.de/). Trees were inferred from GBDP distances computed from genome sequences with FastME 2.1.6.1 (Lefort et al. 2015). Branch lengths are scaled according to the GBDP distance formula d5. The numbers above branches are GBDP pseudo-bootstrap support values > 60% from 100 replicates, with an average branch support of 98.5%. The tree was rooted at the midpoint (Farris et al. 1972)

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