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Fig. 5 | Phytopathology Research

Fig. 5

From: Reverse transcription-recombinase-aided amplification and CRISPR/Cas12a-based visual detection of maize chlorotic mottle virus

Fig. 5

Sensitivity assay for RT-RAA-CRISPR/Cas12a detection. a The RAA products (amplicons) from tenfold serial dilutions of MCMV cDNA was applied to RT-RAA-CRISPR/Cas12a detection. Tubes 1–8, tenfold serial dilutions (100–10–7) of MCMV cDNA (the reverse transcription reaction was initiated with 2000 ng of total RNA); Tube 9, ddH2O as a blank (negative) control. b Results of agarose gel electrophoresis of the PCR products. Lane M, DL2000 DNA marker; Lanes 1–8, PCR amplicon from tenfold serial dilutions (100–10–7) of MCMV cDNA (the same concentration as in a); Lane 9, ddH2O as a negative control

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Phytopathology Research

ISSN: 2524-4167