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Fig. 3 | Phytopathology Research

Fig. 3

From: An increase in the number of peroxisomes is coupled to the initial infection stage and stress response of Botrytis cinerea

Fig. 3

Construction and phenotype of a B. cinerea strain expressing a peroxisome-localized marker. a Schematic diagram of the gene overexpression plasmid used for transformation of the B. cinerea strain B05.10. b Growth phenotypes of the wild-type strain B05.10 and its derivatives containing GFP-SKL at the indicated times. Bar=1 cm. c, d Colony area and conidia number of strains B05.10 and GFP-SKL-1/2 at the indicated times. The values represent the average values, and the error bars indicate the standard deviations of three plates in one experiment. e Fluorescence signals produced by B. cinerea strains containing GFP-SKL or GFP only (GFP with no SKL tag). A fluorescence microscope with GFP channel and UV channel was used. Bar=10 μm. f Detection of GFP and GFP-SKL proteins expressed in B. cinerea by western blot assay using GFP antibody. All experiments were repeated three times with consistent results

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