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Fig. 5 | Phytopathology Research

Fig. 5

From: An increase in the number of peroxisomes is coupled to the initial infection stage and stress response of Botrytis cinerea

Fig. 5

Induction with exogenous oleic acid increases the number of peroxisomes in B. cinerea hyphae. a Fluorescence of B. cinerea strain GFP-SKL cultured in the indicated medium, including YEPD medium or medium with glucose, fructose, or oleic acid as the sole carbon source. Bar=50 μm. The red arrows indicate the AP-like structure. b, c Column charts showing the statistics of hyphal length and peroxisome number. The values represent the average values, and the error bars indicate the standard deviations of 20 hyphae in one experiment. d Column chart showing the statistics of the AP-like structures. The values represent the average values, and the error bars indicate the standard deviations of 3 groups of hyphae under microscopic fields in one experiment. Each group contained 3–4 independent microscopic fields, with a total of approximately 50 hyphae. e Relative expression level analysis of 6 DEGs annotated to the peroxisome pathway via RT-qPCR. BcTubulin was used as an endogenous control, and the sample collected from 10% YEPD medium at 6 hpi was used as a sample control. The values represent the average values, and the error bars indicate the standard deviations of 3 technical replicates in one experiment. The different letters (a, b, c, and d) indicate significant differences at the level of P<0.01. All experiments were repeated three times with consistent results

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