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Fig. 6 | Phytopathology Research

Fig. 6

From: An increase in the number of peroxisomes is coupled to the initial infection stage and stress response of Botrytis cinerea

Fig. 6

The invasive ability of B. cinerea is positively correlated with the activation of the peroxisome pathway. a B. cinerea hyphae in onion epidermal cells at 12 hpi after stained with trypan blue. Bar=100 μm. b Scatter plot showing the percentage of invasive hyphae in a. The different letters (a, b, and c) indicate significant differences at the level of P<0.01. c Fluorescence produced by strain GFP-SKL on onion epidermal layers. Bar=50 μm. d, e Column charts showing hyphal length and peroxisome number. In b, d, and e, the values represent the average values, and the error bars indicate the standard deviations of 20 images in one experiment. f Relative expression levels of 6 DEGs annotated to the peroxisome pathway. The onion epidermal cells or cellophane were inoculated with conidial droplets in 10 mM glucose, and then collected at the indicated times for RT-qPCR analysis. The Y-axis represents the log2 value of the relative expression level. The expression level of each gene in conidia was set as ‘1’ and used as sample control. BcTubulin was used as an endogenous control. The values represent the average values, and the error bars indicate the standard deviations of 3 technical replicates in one experiment. All experiments were repeated three times with consistent results

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